Novel Method to Expand and get Higher Concentration and Proliferation of Chicken Embryonic Fibroblast Cells

Nahmias Yaakov, HUJI, School of Computer Science and Engineering, Bio-Engineering


Chicken embryonic fibroblasts (CEFs) are commonly used for the production of vaccines and are a source material for cultured meat. Fibroblasts are contact-dependent limiting their large-scale production to carrier beads capable of reaching only 5-6 million cells/ml.

Our Innovation

We developed a method that allows the culture of chicken embryonic fibroblasts (CEF’s) in suspension. We produced a sub-clone of the commonly used DF-1 line which can uniquely reach extremely high cells densities in suspension cultures. The cell line is a continuous line of chicken embryo. The cells are free of endogenous sequences related to avian sarcoma and leukosis viruses and have normal fibroblastic morphology. The cells have higher proliferation rate and also can be sub-cultured infinite number of times.  The expected increase in yield is up to 40-fold in comparison to current growing methods

Applications for use

Chicken Embryonic Fibroblasts (CEFs) are commonly used in the industry, and as so, methods for improving large-scale industrial cell production are of need, specifically for vaccine production

The proposed technology can be used by pharmaceutical and biotechnology companies to develop human and veterinary viral and therapeutic vaccines.

Contact for more information:

Mel Larrosa
VP Business Development Healthcare
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