Development of 2,4-D Resistant Non-Transgenic Solanaceae Plants

Mosquna Assaf, HUJI, Faculty of Agricultural, Food and Environmental Quality Sciences, Plant Sciences and Genetics
Rubin Baruch, HUJI, Faculty of Agricultural, Food and Environmental Quality Sciences, Plant Sciences and Genetics





Dichlorophenoxy acetic acid, 2,4-D, herbicide resistant, plants

Current development stage

General list: TRL2 Technology concept formulated

Collaboration Opportunity

Sponsored Research with an option to License Research Results



2,4-D is a herbicide widely used for eradicating broad leaf weeds with a worldwide market value of over $1 billion per year. Despite its success, this herbicide is not used in fields planted with dicots, such as soy, cotton, potatoes and canola. Dicot plants are extremely sensitive to 2,4-D and easily suffer from severe yield drag when exposed to drift and therefore 2,4-D is not used in fields planted with dicots.

To date, a transgenic approach can be used for imparting 2,4-D resistance in plants (both monocots and dicots), however, the path to deregulation with this transgenic approach is costly, time consuming and plant resistance to 2,4-D is negatively influenced by environmental conditions. This resistance to 2,4-D is reduced because the bacterial enzyme used for breaking down 2,4-D has a reduced efficiency in high temperatures.  

Our Innovation

The researchers are developing a way for solanaceae plants of interest to resist 2,4-D herbicide. The novel method is considered a non-transgenic approach and can be used either alone or in conjunction with other known approaches which will enhance the resistance even further.


The unique proprietary approach utilizes CRISPR/CAS to prevent 2,4-D herbicide uptake into the solanaceae plant.


Our approach offers the industry a technology that is non-transgenic which may not need to go through the deregulation process. Furthermore, dicots modified by this approach will be able to benefit from the use of 2,4-D in the field.

The researchers is seeking funding for further research with an option to license the research results.

Contact in Yissum 



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