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"Thaw & Go" Cryopreservation of Adherent Cells

Braslavsky Ido, HUJI, Faculty of Agricultural, Food and Environmental Quality Sciences, Biochemistry, Food Science and Nutrition
YASHUNSKY Victor, HUJI, Faculty of Agricultural, Food and Environmental Quality Sciences
Bahari Liat, HUJI, Faculty of Agricultural, Food and Environmental Quality Sciences, Biochemistry, Food Science and Nutrition
BEIN Amir, HUJI, Faculty of Agricultural, Food and Environmental Quality Sciences, Biochemistry, Food Science and Nutrition

“Thaw and Go” Better Cryopreservation of Adherent Cells

 

Category

Life Science & Biotechnology

Keywords

freeze, thaw, cell culture, cryopreservation

Current development stage

General list: TRL4 Technology validated in lab             

Collaboration Opportunity

Sponsored Research with an option to License Research Results


Background
Cell culture is routinely used in research and is vital in the pharmaceutical industry and in the clinic. Successful cryopreservation is a key feature for keeping original cellular viability and functionality after a long period of storage. In common practice, cells are cryopreserved, dispersed in solutions and are sub-cultured on the appropriate substrates after thawing. These procedures involve time-consuming and expensive steps such as detaching adherent cells from substrates and many medium exchanges. Currently, there is no proven method for successful cryopreservation of adherent cells. In addition, many cell types (e.g., various types of stem cells) are considered unsuitable for cell culture due to the inability to cryopreserve them. This is a bottleneck in cell-based research, therapies, and diagnostics. 

Our Innovation
The research demonstrates the use of a novel technology in which precise regulation of the thawing process enables high rates of post-thawing viability (80%) with significantly more attached cells to the substrate. The results will allow new techniques in the handling of adherent cells to a substrate, 3D scaffold with cells and organoids without the need to detach the cells from the surface. This technology can be an integral preservation method of future lab-on-chip applications to support cutting edge medical research and clinical therapy applications. Our approach will:

  • Enable 80% post thawing viability
  • Reduce time
  • Reduce costs
  • Reduce environmental wastes

 pic_4237.png

Representative image showing the effect of thawing on the Actin through immunofluorescence assay. A; Experiments indicated a distortion of the Actin structure due to water bath thawing. The white arrows are used to illustrate the shrunk cytoskeleton and distorted structure. B; image indicates the no sign of distortion in Actin filaments due to IR assisted thawing. 

Opportunity
The technology has a patent application PCT/IL2017/051313 and needs an industry partner to be developed and commercialized.

 

 

Contact for more information:

Ilya Pittel
VP, BD AGTECH, FOODTECH, VETERINARY & ENVIRONMENT
+972-2-6586693