Highly Sensitive Method for DNA Detection of Viral and Bacterial Infections

Willner Itamar, HUJI, Faculty of Science, The Institute of Chemistry


The technology enables an affordable and easily deployable method for detection of water/soil contamination, and/or rapid detection of viruses in humans and animals.

The  method  employs the extension of the isothermal rolling circle amplification (RCA) process to a RCA-stimulated dendritic synthesis of catalytic nucleic acids (DNAzymes) sensing platform.


The method is based on the improvement of the RCA sensing methods by two elements:

  • The introduction of a functional hairpin that regenerates the analyte and allows the dendritic branching of the RCA chains.
  • The isothermal autonomous formation of DNAzymes in the RCA chains.

These catalytic labels enable simple detection of the chemical constituents of interest.  By the tailoring of different circular DNA templates that lead to the formation of different RCA-branched DNAzyme reporter catalysts, the multiplexed analysis of different analytes is demonstrated.

Applications for use:

This method enable simple and sensitive detection of Bacterial/viral water contamination and/or rapid detection of viruses in humans as well as in animals.

Patent Status

Granted US 9,809,846

Contact for more information:

Mel Larrosa
VP Business Development Healthcare
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