Generation of Insulin Producing Cells from Induced Pluripotent Stem Cells

Benvenisty Nissim, HUJI, Faculty of Science, The Alexander Silberman Institute for Life Sciences



LifeSciences and BioTechnology   


Stem cells, small molecules, Diabetes, cell replacement therapy

Current development stage


Our Innovation

A method of generating insulin producing cells, by dedifferentiating induced pluripotent stem cells (iPSCs). The beta cell-derived iPSCs (BiPSCs) maintained open chromatin structure at key beta-cell genes, together with a unique DNA methylation signature that distinguishes them from other PSCs.

BiPSCs also demonstrated an increased ability to differentiate into insulin-producing cells both in vitro and in vivo, compared with ESCs and isogenic non-beta iPSCs.


  • This method demonstrates an advantageous potential for cell replacement therapy for developing advanced treatments for diabetes.


Human induced pluripotent stem cells (HiPSCs) appear to be highly similar to human embryonic stem cells (HESCs). The researchers demonstrate the generation of iPSC lines from human pancreatic islet beta cells using two genetic lineage-tracing systems.  These reprogrammed cells acquired markers of pluripotent cells and differentiated into the three embryonic germ layers.



Fig 1 Schematic representation of two dual lentiviral lineage-tracing systems for specific labeling of human beta cells.

Patent Status

Granted US 9,394,523

Contact for more information:

Ariela Markel
VP, Business Development, Healthcare
Contact ME:
All projects by:Benvenisty Nissim (3)