Fresh Fish Spawning Agent, Zagin2

Levavi-Sivan Berta , HUJI, Faculty of Agricultural, Food and Environmental Quality Sciences, Animal Sciences



Agriculture, Aquaculture, Fish Culture, Animal Sciences


Spawning, carp, aquaculture

Current Development Stage

General list: TRL4 Technology validated in lab    

Collaboration Opportunity

Sponsored Research with an option to License Research Results

Carp represents 40% of the world aquaculture production of fish, crustaceans, and mollusks. In 2015, global culture carp production reached 28.8 million tonnes, with more than 20 million tonnes produced in China (link) According to FAO projections, it is estimated that to maintain the current level of per capita consumption, global aquaculture production will need to reach 80 million tones by 2050.Induced spawning in fish has traditionally been carried out by a combination of Gonadotropin Releasing Hormone (GnRH) analog, and a non-FDA approved dopamine receptor inhibitor, or alternatively by a calibrated carp pituitary extract (CCPE).

Our Innovation
We developed ZAGIN2, a novel spawning agent which utilizes FDA approved materials and is used by intraperitoneal or intramuscular injections. The agent can be used in most freshwater fish to efficiently increase spawning, specifically in fish that have dopaminergic inhibition on LH release. The agent has an advantage over commercial spawning agents, such as ZAGIN1 (Drori et al. 1994), Ovaprim, Ovatide which include non-FDA approved component for veterinary use.

We found a substance that is approved by the FDA for veterinary use. Together with an already known compound, it is as efficient as the current spawning induction agent. It also showed to be more efficient in increasing the spawning induction hormone (LH) levels in female carp. We named this novel fish agent ZAGIN2.



The ZAGIN2 is more efficient than ZAGIN1 in terms of increasing the spawning-induction- hormone (LH) levels in female carp.

In comparison to commercially available Zagin 1, hatching was significantly improved using ZAGIN2.


We are looking for sponsored research funding to test ZAGIN2 in the lab and at spawning facilities. The ZAGIN2 will be eligible to receive FDA approval and can be used on fish intended for human or animal consumption, unlike ZAGIN1, Ovaprim and Ovatide which are prohibited. The ZAGIN2 can be reformulated as a feed additive in future development.

1. Drori S., Ofir M., Levavi-Sivan B. & Yaron Z. (1994) Spawning Induction in common carp (Cyprinus Carpio) using pituitary extract or GnRH superactive analog combined with metoclopramide - analysis of hormone profile, progress of oocyte maturation and dependence on temperature. Aquaculture 119, 393-407.




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